Part:BBa_K209447:Design

pEF1-SSFYFP-Rs1.3-ActA(30-612)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 346
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3710
  Illegal PstI site found at 346
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 128
  Illegal BamHI site found at 1952
  Illegal BamHI site found at 2166
  Illegal BamHI site found at 2547
  Illegal XhoI site found at 1479
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 346
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 346
  Illegal NgoMIV site found at 3578
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 84
  Illegal SapI.rc site found at 2328
  

Design Notes

Cloned into pEF5/FRT/V5-DEST.